FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26707482
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA37500.029.25329850
TATCGCG7750.028.93150922
GCGTCGT42750.027.67643412
CATACGA20900.027.56030815
GCCGTAT42800.026.2750248
CGCCGTA43500.026.08981347
GATCGGA51700.024.9009192
CGGCATA24850.023.5979712
ATCGGAA64450.021.152813
GCATACG30050.020.89058314
GTCGCCG53850.020.75566545
CCGTATC55650.020.58015449
AGAGCGT61250.019.5422579
TGGTCGC54450.019.32386443
AGCGTCG61200.019.2764411
ATACGAG28800.018.20395716
TCGCCGT61300.017.67020846
GGTCGCC64750.017.52755244
TCGTGTA67150.017.51548815
AGATCGG68550.017.3706191
Produced by FastQC (version 0.11.5)