FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28570957
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT80750.037.63921442
ACACGTC97900.032.1680913
TATGCCG97250.031.60637746
GATCGGA99850.031.1015551
ACTTAGG105150.030.0203132
ATGCCGT101200.029.89517847
TCTCGTA101450.029.61981841
CACTTAG110400.028.6851531
CACGTCT110200.028.5776414
TTAGGCA111400.028.45981834
CTTAGGC112350.028.18841633
CACACGT118100.027.10411812
CGTATGC117400.026.38701844
ATCTCGT116500.025.76382340
ACGTCTG124800.025.34498215
TCGGAAG129150.024.4953883
TCACTTA132600.024.01274130
CGTCTGA135250.023.51419316
TCGTATG134400.023.1785643
GCCGTCT136250.022.2300349
Produced by FastQC (version 0.11.5)