FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35271828
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT12600.010.5556721
CGCGAAA17300.010.317945543
CGAAGAT122500.010.0286831
TCGCTCG47400.09.74683314
TCGGGTC65550.09.6644572
CTCGGAT84000.09.6251061
CCGGGAT76250.09.5476461
TCGCGCG8555.456968E-129.4150956
CTCGGGT83950.09.213921
CGCCAAT64800.09.1281881
CGCGTAT6553.4598997E-89.084071
CGCGGAT32550.09.0323581
CGCGGGT28100.08.9680711
CGTTCGC26550.08.832376516
CGTATCG25600.08.7499853
CTCGGTT47300.08.6576011
CCGGCAT59650.08.5667331
CGCAGAC92100.08.4365761
CCCGTAT42100.08.3967681
CGCGTTC29200.08.27054614
Produced by FastQC (version 0.11.5)