FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26120466
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG43900.033.0825743
TATGCCG48700.029.82728846
CGTATGC50000.029.05049744
GATCGGA50050.027.2328781
ACACGTC57350.027.1296813
CTCGTAT57350.025.14439242
ACGTCTG68250.022.99907315
ATCGGAA60150.022.713912
CACGTCT72600.021.95366114
TCTCGTA68150.020.60282741
ATCTCGT71250.019.9968240
ATGCCGT79850.018.66678247
TCGGAAG80950.017.1758753
GCACACG95400.016.92384511
CACACGT93150.016.81413712
GCCGTCT89700.015.84763849
CCGTCTT96300.015.01222450
TTACACG30700.014.94490832
AATCTCG39000.014.8607439
TGCCGTC105600.014.4090248
Produced by FastQC (version 0.11.5)