FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26120466
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG6800.018.26130720
TTACACG29900.013.9617232
CTCGTTT42450.012.4385091
CGTATCG18800.011.7483683
CTCGGTT31850.011.7022381
CGAAGAT92650.011.43531
TCGCTCG28500.011.37663314
TAAGTCG22300.011.29161920
GTATCGA24550.011.2458924
CTCGGGT54750.011.0938711
CTCGGAT63900.010.8014921
CTGGAAT205750.010.6509531
TCGGGTC46650.010.5789642
CTCGAAT53500.010.1274591
CTCGTAT28950.010.0134871
CTCAAAT163450.09.9869021
CTCAGAT150800.09.8177181
CTCCGAT49050.09.779811
TAAGCGA20250.09.710587546
CTCGACT29850.09.4803441
Produced by FastQC (version 0.11.5)