FastQCFastQC Report
Sat 11 May 2024
EGAF00004858921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15506380
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGNTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA573630.3699316023469049TruSeq Adapter, Index 18 (97% over 40bp)
GANCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT416530.26861846543164813TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph