FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15506380
Sequences flagged as poor quality0
Sequence length75
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGNTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA284320.18335678604548578TruSeq Adapter, Index 18 (97% over 40bp)
GANCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA167110.10776854430240972TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG83200.091.217671
GATCGGA115650.068.31982
ATCGGAA122000.064.490573
TCGGAAG197950.036.7161834
TATGCCG193000.036.21832349
TCGTATG192000.035.97083346
CGTATGC195400.035.90389647
ATGCCGT209000.033.4107950
CTCGTAT206650.033.2093545
CGATCGG6700.032.7233961
ACGTCCG220300.032.52897633
CGTCCGC220100.032.49793634
AGTCACG226900.032.04717329
CGCACAT217450.031.91382238
ACACGTC225450.031.87126414
GTCCGCA224450.031.70752335
GTCACGT228350.031.43963230
TCACGTC231050.031.31332831
TGCCGTC221300.031.30691151
ACGTCTG231150.031.21291416
Produced by FastQC (version 0.11.5)