FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15506380
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA136900.052.34494450
GCCGTAT142100.051.24442348
AGATCGG147250.051.0748061
GTCGCCG142500.050.7664845
GCGTCGT144600.050.68248712
CGCCGTA142800.050.39681247
TGGTCGC138850.049.52052743
TCGCCGT141550.049.443846
CCGTATC148100.048.7766649
TCTCGGT146300.048.37319637
CGGTGGT143450.047.46868540
CGTCGTG157050.046.82188413
AGCGTCG156900.046.7664411
TCGGTGG150900.046.68551339
GGTCGCC156300.046.24055544
AGAGCGT161300.045.5330669
GAGTGTA141800.045.35911628
GTCGTGT160250.044.95013414
GATCGGA169850.044.9200132
GTATCAT159700.044.87177351
Produced by FastQC (version 0.11.5)