FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19699132
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51450.028.8423743
TATGCCG55400.026.97236446
GATCGGA51650.026.8646871
CGTATGC57500.025.86752944
GTCACGA59450.025.253629
AGTCACG62800.023.96251928
ACACGTC65650.023.44405713
ACGATCA65050.023.2356132
ATCGGAA61150.022.5988462
CACGATC67850.022.27673131
ACGTCTG70050.022.26628915
CTCGTAT66150.021.8083742
ATCTCGT70350.020.50622240
CACGTCT76550.020.2857114
TCACGAT74550.020.27466430
CGATCAG77000.019.62991333
CACACGT81350.019.04571512
ATGCCGT79950.018.862347
TCTCGTA75050.018.76340541
GCACACG88500.017.77932411
Produced by FastQC (version 0.11.5)