FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29466149
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG77300.048.5108243
CGTATGC81600.045.9544944
TATGCCG85800.043.3833146
ACACGTC93800.041.3468713
GATCGGA96100.040.052171
CTCGTAT90000.039.6720742
CACGTCT110850.035.4230314
ATGCCGT112750.032.70773347
CACACGT123900.031.69203812
TCGGAAG124000.031.1885573
TCTCGTA125500.028.53255841
GATCTCG123900.028.45557439
GCCGTCT134000.027.46952249
GTATGCC140900.026.93210245
TGCCGTC137850.026.72721748
GCACACG156150.026.03147311
GTCACAC148000.025.75593229
ACGTCTG155800.025.08674615
CGTCTGA170350.023.02483616
AGTGATC153800.022.81138636
Produced by FastQC (version 0.11.5)