FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40298816
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG58800.013.15283543
TCGCTCG46150.011.37264414
TATGCCG71200.011.20675346
CGTATGC74750.010.62720644
CGTATCG30900.010.530963
CGCGAAT20700.010.49541
CTCGTAT82250.010.33891142
TATCTCG77900.010.24574639
CGCGGGT28450.010.0997181
CGAAGAT134150.09.9781571
CTCGGTT52900.09.8035581
TTACACG42750.09.58287132
CGCAGAC92300.09.3771941
CGACGAT30850.09.3140031
CCCGTAT48700.09.2819331
CTCGGAT93400.09.2667561
CTCGTTT60300.09.1815811
GTATCGA42200.09.1204154
CGCTCGT56900.09.10104715
TATATCG23900.09.08434535
Produced by FastQC (version 0.11.5)