FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38638826
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA46150.020.3997949
GCCGTAT52450.018.48338947
GATCGGA80150.016.85521
GCGTCGT63800.014.812489511
ATCGGAA92300.014.5228452
GTCGCCG62950.014.34416544
AGCGTCG80550.014.03523510
CGCCGTA68150.013.91718346
CGCGAAA15600.013.68510243
AGAGCGT90500.012.6077548
CCGTATC77600.012.22257648
CCCGTAT49500.011.10055351
AAGAGCG118200.011.0747397
CGTATCG35800.010.8519433
GTAGATC94800.010.70594931
CGTTCGC28350.010.49418316
CGCGGGT30800.010.4541151
TGGTCGC74800.010.43395342
TCGGAAG131000.010.3129663
GATCTCG126050.010.3007934
Produced by FastQC (version 0.11.5)