FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30379828
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61450.021.72644243
CGTATGC63900.021.37931644
GCGTCGA40750.020.6570111
CGTCGAT41050.020.50607712
GCGTAGC55350.020.19427710
TATGCCG69650.019.5152746
AGTGTCG55400.019.30418451
TATCTCG69650.018.97042839
GTGCGTT60450.018.14806641
CTCGTAT78150.018.14313542
CGTTCGA61250.017.96735244
CGATGTA75950.017.66955434
GTGTCGA63050.017.23554252
ACCGATG81700.016.84824232
AGTGCGT64650.016.80898540
TCGCTAT94100.016.16778828
ATGCCGT83500.016.0717347
CACCGAT84850.015.97883931
ACACGTC86400.015.89187913
GATCGGA82300.015.6657531
Produced by FastQC (version 0.11.5)