FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22586813
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA289640.12823411607472024TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA256470.11354855596493404TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG130150.036.3072471
TCGTATG190750.028.03945245
TATGCCG194050.027.6679548
GATCGGA203500.027.5461481
CGTATGC199150.026.99362246
ATCGGAA213900.026.3110772
ACACGTC214000.026.18896513
CTCGTAT198150.025.72017344
ACGTCTG217700.025.68120215
GCACACG218500.025.17073811
CACGTCT226550.024.8885814
ATGCCGT214850.024.87837649
TCTCGTA202050.024.83592243
AATCTCG204050.024.60907741
TCGGAAG228800.024.499643
GCCGTCT220550.024.23540751
CACACGT224700.024.21693412
ATCTCGT207900.024.0714242
CCGTCTT226050.023.79644452
TGCCGTC228200.023.45291950
Produced by FastQC (version 0.11.5)