FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36048237
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG56600.022.3087743
CGTATGC62350.020.52808444
TATGCCG63150.019.939846
CTCGTAT69750.018.10296242
ACACGTC74550.017.5848913
TATCTCG65650.015.502439539
GATCGGA71600.015.2759551
AGTCACG64850.014.94852328
ACGTCTG92350.014.90520515
CACGTCT94000.014.38666514
TCTCGTA92050.013.71734441
AGATCGG72750.013.1848131
ATGCCGT104500.012.47891547
GTCACGC79550.012.22956129
ATCTCGT108600.011.72220540
CACACGT115400.011.65903312
TTACGCG15650.011.46285354
CGCCAAT87250.011.347959533
ATCGGAA99400.011.074092
TCGGAAG108850.010.7784283
Produced by FastQC (version 0.11.5)