FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36048237
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA39600.023.4314650
GCGTCGT44550.022.87719312
GCCGTAT47800.019.70409248
CGCCGTA54350.017.96208647
GATCGGA58250.017.7367232
AGAGCGT65800.015.436939
AGCGTCG70150.014.5776311
AGATCGG72150.014.2207151
TTACGCG16450.014.21053954
GTAGATC72400.013.95749932
CCGTATC70650.013.42558149
TGGTCGC74900.012.80443443
TCGTGTA84400.012.64682915
CGTCGTG84950.011.99761513
ATCGGAA84800.011.9960673
CGCGGAT28650.011.9790511
GGTCGCC84800.011.7960144
CGTGTAG90250.011.67456816
GTATCAT85800.011.57554151
GTCGTGT93250.011.55743614
Produced by FastQC (version 0.11.5)