FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24765389
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA371250.14990679128843887TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG365850.048.092143
GATCGGA386650.047.9092641
TATGCCG371450.047.53232646
CGTATGC373750.047.27643244
CTCGTAT367500.046.1394142
ACACGTC394900.046.1077713
ATCGGAA399850.045.8752822
CCGTCTT363350.045.8080750
GCCGTCT363000.045.73687449
ACGTCTG404250.045.14262415
ATGCCGT391100.045.02082447
TCTCGTA370600.044.89686241
CACGTCT409800.044.57288414
TGCCGTC374550.044.05254748
ATCTCGT376950.043.9323340
CACACGT418350.043.7271812
TCGGAAG419050.043.6730963
GCACACG430450.042.83094811
CGTCTGA434200.041.9579116
CGTCTTC398350.041.74503751
Produced by FastQC (version 0.11.5)