FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24765389
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA35900.044.47931715
GCATACG46050.039.0767614
CGGCATA45400.038.90331312
ATACGAG46700.033.08478516
CGTATCA105900.032.86620750
GCCGTAT113700.030.60986548
CGGCGTG65000.030.35674512
CGCCGTA118650.029.47805447
TGGTCGC118800.029.22412943
TCGGGGG160050.028.93554338
TCGCCGT120550.028.04185546
CCGTATC124950.027.96543149
AGATCGG133700.027.8907031
GTCGCCG127850.027.81789245
AAGACGG104150.026.9527668
GGCGCCG87600.026.7629144
AGACGGC104850.026.7417169
GCGTCGT135850.026.68797312
AGCGGCG76350.026.65246610
GTATCAT140700.025.32568251
Produced by FastQC (version 0.11.5)