FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35715596
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT20550.012.1128371
CGACCGT11650.011.4355481
CGCGAAA13250.010.83072943
TACCGTA31300.010.5101029
TATGCCG37850.010.26479946
ACCGTAT30200.010.08174510
CCCGTAT37050.010.030371
CTCGGAT83600.09.7712241
TCGTATG40800.09.52253343
CGTATGC41700.09.48500444
CTCGGGT74000.09.3806471
CCGTATG30800.09.317224511
CGACCTA31250.09.1830564
CGTATCG30150.08.9377223
CTCGTTT51200.08.9016941
TCGGGTC63450.08.7146282
CTCGGAG116900.08.6972671
CGCGCTA13850.08.59255846
CGACCGA26350.08.50144446
CTCGGTT38150.08.3626931
Produced by FastQC (version 0.11.5)