FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58565297
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG214500.032.3920543
CGTATGC222000.031.37542544
TATGCCG225100.030.63634546
CTCGTAT244150.028.24644942
ACACGTC252450.028.21958413
TCTCGTA267850.025.50241741
GATCGGA234650.025.409311
CACGTCT284750.025.06678214
ATGCCGT278650.024.77351647
ACGTCTG287450.024.77132415
ATCTCGT289500.023.64290840
CACACGT304600.023.52407312
CGTCTGA328400.021.84002916
GCACACG345550.021.0757611
GCCGTCT328650.020.98366549
TGCCGTC338650.020.60840848
GATCTCG318950.020.60535839
ATCGGAA293050.020.2771762
GTATGCC345950.020.19348745
AGATCGG180750.019.757491
Produced by FastQC (version 0.11.5)