FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36339353
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG87700.026.659245
CGTATGC98450.023.92597446
TATGCCG98050.023.77370648
GATCGGA104700.021.8322121
AGTCACG109850.021.50650428
TGAAACG111750.021.48521435
ACACGTC114900.020.68296813
GTCACGT118400.020.21950329
GAAACGA128450.019.75454336
ACGATCT112950.019.67665139
ACGTCTG124700.019.19785915
CTCGTAT119700.018.94746844
ATCGGAA125100.018.3816932
CACGTCT133100.018.1177514
AACGATC126750.017.72763438
ATCTCGT124400.017.72519742
AAACGAT140550.017.58075737
CGTGAAA140500.017.31301733
AGATCGG90800.016.8857171
TCTCGTA135650.016.07456243
Produced by FastQC (version 0.11.5)