FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33176188
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG39200.013.72962214
CGCTCGT46650.011.31561615
CGAAGAT103300.011.2190871
TAAGCGA20900.010.23104746
CGCAGAC85150.09.6407521
CGGAGAT85350.09.4160971
CGTATCG21250.09.4108573
CAATCGG41100.09.1441794
TCGTGCG57850.09.12233424
CGCGGGT24300.09.0843171
ACTCGTG57800.09.0096822
CGGACAT60000.08.9104371
CGCCAAT50300.08.8458641
CGACAAT42000.08.78725051
CGACCGT13800.08.7479731
CCCGATA59850.08.70661769
GTATCGA32100.08.70041754
CTCGGGT63350.08.6570331
CTCGTAC62300.08.63915117
CTCGGTT40450.08.6123441
Produced by FastQC (version 0.11.5)