FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26264740
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT28350.022.01817312
CGTATCA29200.021.27027550
GATCGGA35200.018.4302022
TATCGCG16350.017.7513322
CGTATCG23450.017.5113263
GCCGTAT37400.016.97561648
CGCCGTA41200.015.15868347
CGCGTAA20300.014.7889771
AGAGCGT44150.014.6080819
CCCGTAT27000.013.675241
AGCGTCG47450.013.44607211
GTATCGA23300.013.3291224
GCGGTAT46600.013.2550711
CCGTATC50400.012.59698449
AGATCGG48250.012.5872331
GTCGTGT50450.012.57783214
CGTGTAG51600.012.49639616
TCGTGTA53000.012.49253315
CGTCGTG55750.012.24828413
GTAGATC55850.011.79664932
Produced by FastQC (version 0.11.5)