FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30693949
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT60200.09.94255351
CGCGGGT26550.09.8876951
CGTATCG21300.09.8596663
CTCGGAT72550.09.6491921
CTCGGGT62100.09.3001661
CTCGGTT40600.09.2247811
CTCGACT42600.08.9560231
CGCGTTC22650.08.80787214
CGAAGAT98350.08.7550581
CGCGGAT25300.08.71602251
CGTTCGC21450.08.64788916
CGCGAAT12250.08.5720261
CGCGTAA5754.6205423E-68.5223331
GTATCGA28850.08.3713174
TCGCTCG33750.08.19252314
CGAGAAT55750.08.16200351
CGGAGAT100000.08.0155581
CGCGAAA11901.8189894E-127.940928543
TCGGGTC46150.07.88787362
CGGGAAT52850.07.88134431
Produced by FastQC (version 0.11.5)