FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34374754
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG36200.014.67623614
TCGTATG47550.013.85747545
CGCGTAT4551.03682396E-1012.9152051
TATGCCG53600.012.29333948
CGTATGC53650.012.153275546
CGCGGGT22400.011.8824631
GTATCGA34600.011.6675564
CGTATCG27550.011.5230973
CGCTCGT45950.011.48704815
CCGGGAT62650.010.3177331
CGGGGAT59600.010.2657531
CGCAGAC78650.010.2404971
CCCGTAT40900.010.2264731
CGATCCA46750.09.8161274
ACTCGTG56900.09.76146222
CGAAGAT111000.09.591611
TTACACG34550.09.38602532
CTCGTAT71200.09.25453644
TATATCG21850.09.15753235
CCGTATC41600.09.1251552
Produced by FastQC (version 0.11.5)