FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20888737
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA221010.1058034289004644TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG159450.029.30830445
AGTCACG176200.027.2964128
CTCGTAT158050.027.26196544
ACACGTC177150.027.22850213
TGAAACG174600.026.92213435
GATCGGA185650.026.4382951
GTCACGT180000.026.39914529
TATGCCG178950.026.34301648
ACGTCTG183600.026.29049915
TCTCGTA159600.026.14353643
CGTATGC181350.025.9943946
GAAACGA183850.025.9371636
AGATCGG123400.025.8718971
ACGATCT160000.025.63093839
ATCGGAA192550.025.5740592
CACGTCT190950.025.40340614
GCCGTCT186200.025.1893251
ATGCCGT186350.025.11420849
CCGTCTT186250.025.07282852
ATCTCGT165750.024.98837742
Produced by FastQC (version 0.11.5)