FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20888737
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA34950.039.5113715
CGTATCA77650.039.15656750
CGGCATA36450.037.35675412
GCATACG36750.035.79570414
GCCGTAT94500.032.3884248
GTCGCCG98500.030.81079345
CGCCGTA99900.030.81045747
GCGTCGT102800.030.29986412
GTATCAT103350.029.55548151
ATACGAG44100.029.51881216
CCGTATC104100.029.37203849
TGGTCGC100900.029.26051543
TCGCCGT102100.028.79800446
AGCGTCG115950.027.01293811
AGATCGG116800.026.8236181
TCTCGGT120900.025.65870937
GATCGGA125100.025.511812
GTAGATC122500.025.3920932
GGTCGCC120400.025.29403144
CGTCGTG126050.025.04130413
Produced by FastQC (version 0.11.5)