FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12398400
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA120900.061.31405612
CATACGA99050.060.98005315
GCATACG119000.060.17850514
ATACGAG97550.056.46166216
AGACGGC162350.056.3774769
AAGACGG164150.055.6008038
ACGGCAT133450.055.5998811
GACGCGT17650.053.4697945
ACGCGTG16750.053.0520746
GACGGCA140300.052.90955710
CGGCGTG62300.051.20112
GGCATAC147700.050.61123313
GAAGACG185750.049.4151657
ACGGCGT34250.049.19878411
TACGAGA79700.048.3924617
ACGAGAT78450.047.0995218
ATACGCG5950.046.34225516
AGCGGCG45250.041.9125310
CGCGTGC25800.041.90884847
GGACGCG17550.041.60786444
Produced by FastQC (version 0.11.5)