FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33800613
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA22150.018.3686715
CGGCATA28450.017.46180212
GCATACG29100.016.47098414
CGTATCG34200.016.0341993
CCCGTAT39350.014.2848811
GTATCGA36150.014.119694
TTACGCG16650.013.46996654
GCGGTAT52550.012.9935021
ATCGAAG70550.011.8374596
CCGTATC58500.010.7886942
TACGCGA21250.010.71354855
ATCGCGC41700.010.59449867
TATCGAA50550.010.4365375
GCGCTAA20500.09.7606342
TAGCGAC24700.09.50234226
TCGAAGG91550.09.4613747
GCGATCG40100.09.4607734
ACGATCG8052.0008883E-119.425473
CATCGCG52850.09.33723566
CGTGCGA40000.09.30837831
Produced by FastQC (version 0.11.5)