FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41065233
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA61600.028.46413250
GCCGTAT70450.025.13679748
CGCCGTA72300.024.92925647
GCGTCGT83950.022.09771712
CCGTATC88350.020.20244849
GTCGCCG85400.019.87539745
GATCGGA97200.019.5898532
TGGTCGC83800.018.62545643
AGCGTCG101150.018.51413511
AGAGCGT112250.016.9340049
GTAGATC111700.016.85641732
ATCGGAA114800.016.587343
TCGCCGT97150.016.57115646
GGTCGCC105400.016.13709844
AGATCGG117300.016.0822371
GTATCAT115200.015.49357951
CGTCGTG122550.015.39335613
TCGTGTA127350.014.50971815
GTCGTGT128150.014.47386214
GAGTGTA121900.014.26851528
Produced by FastQC (version 0.11.5)