FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41546414
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG69000.027.24779945
TATGCCG74500.025.23612648
CGTATGC74600.025.109946
CTCGTAT83400.022.12950744
ACACGTC97200.021.61407313
GATCGGA85200.020.2518621
CGTCCCG94550.018.7629935
ACCCGTC96750.018.07791332
CACGTCT122700.017.6001114
TCTCGTA108100.017.04121643
ACGTCTG125350.017.03538115
GTCCCGA111400.015.95596236
CACACGT135650.015.86902912
TCCCGAT107050.015.76605937
ATGCCGT124050.014.98906149
CCGATCT111600.014.8138239
ATCTCGT124850.014.53391942
AGATCGG83950.014.1818521
CGTCTGA153850.014.12623916
CCCGATC120400.013.78858138
Produced by FastQC (version 0.11.5)