FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25510268
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG88950.042.55053743
TATGCCG93250.040.735346
CGTATGC95150.039.7060844
CTCGTAT98900.037.33556742
GATCGGA102200.035.5324171
ACACGTC111150.034.97515513
TCTCGTA106600.034.57451641
ACGTCTG117350.033.12736515
ATCGGAA110650.032.587122
CACGTCT122800.031.76866714
ATCTCGT116750.031.45093740
ATGCCGT120950.031.29291247
CGTCTGA135850.028.66641616
CACACGT136450.028.66576212
CCGTCTT139350.027.23462150
GCCGTCT139950.027.04444749
TCGGAAG135750.026.775713
GCACACG148400.026.77256211
GATCTCG138450.026.71920639
GTATGCC147200.026.08445745
Produced by FastQC (version 0.11.5)