FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25510268
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA44050.027.80490549
GCCGTAT47750.026.87861347
CGCCGTA48600.026.04405646
GATCGGA78650.024.1457841
GCGTCGT58350.023.47385211
AGCGTCG70050.022.85306710
ATCGGAA84500.022.445112
CCGTATC57050.021.95271548
GTAGATC79500.020.09605431
AGAGCGT79150.019.6578718
CGTGTAG85700.018.71853815
AAGAGCG105050.018.3574757
GTCGCCG66900.018.2044444
TCGGAAG109200.017.589433
TGGTCGC61150.017.32216542
ATCTCGG109150.017.19550535
GATCTCG106850.017.1159134
CGTCGTG81550.017.04966412
TCTCGGT84500.017.02625736
GGTCGCC76250.017.01377343
Produced by FastQC (version 0.11.5)