FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12666483
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA84050.059.9875115
CGGCATA98600.059.29735612
GCATACG96550.059.15118414
ATACGAG75300.054.71801816
ACGGCAT118600.051.33396511
AAGACGG143450.050.741478
AGACGGC142700.050.728679
CGAGATT66350.046.24226419
TACGAGA68550.045.91361617
GACGGCA133200.045.9123510
GAAGACG164800.044.872237
GGCATAC131850.044.71164313
ACGAGAT70350.043.2346218
ACGGCGT29500.040.62779211
TCGGGGG111550.040.3165538
CGGCGTG60500.039.2317712
CAAGCAG233400.035.4695241
TTCTGAC129100.035.2849624
CTCGGGG106050.035.1057537
TGACATG124500.035.0812627
Produced by FastQC (version 0.11.5)