FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30837659
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA42700.040.96128550
GCGTCGT53000.033.6545212
GCCGTAT53550.032.91983848
GATCGGA60900.029.9831962
CGCCGTA63500.027.81577147
AGAGCGT72150.025.1045489
CCGTATC70700.024.83690649
TGGTCGC73700.023.25698343
AGCGTCG76700.023.16589211
AGATCGG76050.022.7393531
GTAGATC78100.022.57190932
GTCGTGT80400.022.57091914
TCGTGTA81150.022.1057615
CGTGTAG82850.021.94026216
CGTCGTG84650.021.7235313
ATCGGAA86200.021.2230683
TCGCCGT82050.021.14848946
AAGAGCG92850.019.8063458
GTATCAT92850.019.58007851
GGTCGCC89450.019.47444
Produced by FastQC (version 0.11.5)