FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22136382
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG64500.042.83374443
GTCACGA65550.042.51390529
AGTCACG68850.040.69782628
CGTATGC70950.038.65553344
TATGCCG71300.038.56023446
ACACGTC73800.038.4871313
GATCGGA70100.037.9908141
CTCGTAT75950.035.18133542
CACGATC88450.031.96748531
CACGTCT89650.031.8326314
TCTCGTA83600.031.55995241
TCACGAT89550.031.29231330
ATCGGAA88300.029.9642
ATGCCGT92150.029.90814647
ACGATCA95250.029.71944832
ACGTCTG96600.029.47273415
TCGGAAG91400.029.1979393
CACACGT103450.027.68189212
ATCTCGT99600.026.35509740
CGATCAG107200.026.34455933
Produced by FastQC (version 0.11.5)