FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33263834
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61150.030.12003143
TATGCCG66600.027.49988746
CGTATGC69000.026.54336744
CTCGTAT79450.023.48619542
TATCTCG81050.022.17138139
ACACGTC86450.021.79851313
ACCGATG85650.021.79217732
GATCGGA87200.021.7068921
TCTCGTA88300.020.8196741
TATCGCG18000.018.58702322
CGATGTA102900.018.3398434
CACCGAT101650.018.29403731
CACGTCT107200.017.89927714
ATCTCGT106950.017.22132340
CCGATGT111950.016.70369133
ACGTCTG121200.015.70941815
ATGTATC117900.015.4464336
CACACGT123500.015.42659612
ATGCCGT121350.015.37687547
TCGGAAG127600.015.3194113
Produced by FastQC (version 0.11.5)