FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33049561
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA15450.017.67216743
CGAAGAT114350.012.1320231
TTACACG42400.012.05372332
CGACCTA18050.012.0177354
CCGCGAA26500.010.30200642
CTCGGAT84100.09.9141431
CGACCGT15200.09.910581
CGTTCGC22600.09.59823416
TAAGCGA21650.09.53977746
CGTATCG26100.09.5180153
CTCGTTT48650.09.3612691
GTATCGA33350.09.3368914
CGCGTAT4158.7833156E-59.2857891
CGCGTTC24150.09.27203214
CTCGGTT40100.09.1731631
TCGCTCG44300.09.16149414
TCGGGTC55800.09.0294522
CTCGGGT76800.08.9862651
CGCGCGT9455.456968E-128.8972051
TCGTATG43700.08.81119843
Produced by FastQC (version 0.11.5)