FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20722775
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44550.026.04709443
CGTATGC47800.024.77591344
TATGCCG47950.024.69828846
CTCGTAT52450.021.9937542
ACACGTC59350.021.51038413
GATCGGA60150.021.4644991
ACGGCTA60100.020.50181232
AGTCACG53600.019.54938528
ACGTCTG66800.019.21294615
ATCGGAA69400.018.7869422
CGGCTAC68200.018.26703533
TCTCGTA60650.018.11979141
ATGCCGT67950.017.73010347
CACGTCT74150.017.35511214
TCACGGC65950.015.68149230
ATCTCGT69000.015.5313340
TCGGAAG85700.015.2932753
CACACGT84000.015.27930912
GCCGTCT77900.015.20260549
TGCCGTC83650.014.60639748
Produced by FastQC (version 0.11.5)