FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37875642
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG51300.014.12202214
CGCGCAT13100.011.7655251
CGCTCGT63100.011.53660115
CGTTCGC29400.011.18985516
CGCGTTC30750.011.153851514
CGAAGAT135100.011.0973251
TCGGGTC69250.011.017382
CGCGAAC9650.010.8898991
TAAGCGA26250.010.7997746
CCGGGAT81100.010.754951
CGACCTA18750.010.6392124
TCGTATG56100.010.29404845
CTCGGAT98500.010.1709081
CGCAGAC100250.010.09818651
CGCGAAA19100.010.07846643
CGCGATT13300.010.0083361
CTCGGGT93400.09.9761891
CGGGGAT71150.09.9450371
CGCGGAT29700.09.9072421
ACTCGTG77250.09.87734722
Produced by FastQC (version 0.11.5)