FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26026671
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG43000.012.7572614
CGCGAAA11100.011.18765443
CGCAGAC78700.010.7071281
CGCCGAT40450.010.5867111
CGCGGAT22550.010.2609161
CCCGATA53550.010.04904169
ACCCGAT54150.09.93771468
CGAAGAT83200.09.7544411
CCGGGAT63350.09.7035641
CTCGGAT64300.09.5601991
CGCTCGT59300.09.31245715
TAAGTCG24950.09.26772720
CGCGTAA4151.0267079E-49.1538221
CTCGAAT43500.09.1299091
CGCCAAT51300.09.0208041
TACTCGT57900.08.88123421
ACTTCGC62150.08.8806911
ACTCGTG60400.08.85290822
CTCGACT32450.08.8332641
GCTCGTA65450.08.6483516
Produced by FastQC (version 0.11.5)