FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23448062
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG49000.024.2197246
CGTATGC52300.022.95537247
TATGCCG53200.022.4369549
CTCGTAT54650.021.58950445
TATCTCG55800.021.20638542
GTCACGA57250.020.36719930
CACGAGT61200.018.77060732
GATCGGA61000.018.7207982
AGTCACG65200.017.93787229
ACACGTC67900.017.88412314
ACGAGTG63400.017.84727533
TCTCGTA67250.017.74968144
TCACGAG71550.016.3928631
ACGTCTG78100.016.12310216
ATCGGAA72150.015.9228293
ATCTCGT75350.015.795835543
AGATCGG69250.015.7112571
ATGCCGT81000.015.07754350
CACGTCT82750.014.79971215
CGTCTGA87650.014.2482217
Produced by FastQC (version 0.11.5)