FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28916975
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30100.027.73642743
CGTATGC39300.021.4202144
TATGCCG39150.020.88541246
CTCGTAT41550.019.42882342
ACACGTC50150.018.23295213
GATCGGA46500.018.1842691
CGTATCG31450.017.9899253
GTATCGA33450.016.7083424
ATCGAAG62300.014.3433376
GTCGCTA22650.014.31569727
CACGTCT66500.013.80214
CCGTATC45000.013.7995892
CCCGTAT38200.013.5522241
GCGGTAT48900.013.5511151
TCTCGTA65400.012.81829341
TTACGCG16250.012.73922754
ACGTCTG77050.011.86441615
CGCGTAA27050.011.7382481
CGACCTA11700.011.4998774
CCGACAT49550.011.4230711
Produced by FastQC (version 0.11.5)