FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26453299
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG5702.5465852E-1111.6664668
CGCGCGT6650.011.5787481
CGCGTTC19450.011.51596714
CGTTCGC19450.010.79597416
CGCGAAA13000.010.76910743
CCGCGTT22000.010.34045813
TCGGGTC46400.010.3341272
CGCGATT8700.010.0572981
CTCGGGT61100.09.8525311
CGCGGAG37300.09.7585461
CGAAGAT86450.09.7164331
AGTCGCG10200.09.6075146
CGCGGAT16800.09.5831681
CGACCTA23450.09.552494
TTACACG30350.09.45580432
GCGTTCG24500.09.42794615
CCGGGAT43900.09.4075821
CGCGAAC6705.007678E-99.4028231
TTCGCAC23150.09.37281518
CCGGCAT42950.09.2897121
Produced by FastQC (version 0.11.5)