FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29793823
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG77150.036.48705743
GATCGGA75650.035.2141231
ACACGTC89600.033.42045213
TATGCCG86550.032.8101746
CGTATGC87700.032.2996744
CTCGTAT89100.031.20700842
ATCGGAA87100.030.2634262
ACGTCTG100500.030.1046915
CACGTCT109450.027.98967214
TCTCGTA105650.026.28579741
AATCTCG76000.024.69318839
ATCTCGT115150.024.50649840
CACACGT130050.023.42345612
ATGCCGT120500.023.25116247
TCGGAAG118750.022.6332043
GCACACG142750.021.79872311
GCCGTCT135300.020.78416449
CCGTCTT135250.020.76612950
CGTCTGA150500.020.2165916
TGCCGTC154150.018.5336248
Produced by FastQC (version 0.11.5)