FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29793823
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA21050.024.41494215
GCATACG26450.020.47417614
CGGCATA27150.018.93370812
ATACGAG32600.015.44744916
CTCGGGT71800.012.3525831
TTACACG39750.012.32490632
TCGCTCG39250.012.30327914
CGACCTA14500.011.9002334
CGCGTAA3501.2349992E-611.8321121
CGAAGAT100600.011.732141
CTCGTTT46900.011.6260811
TAAGTCG34100.011.53148120
CTCGGAT73350.011.1035281
CGTATCA25400.011.00210250
TCGGGTC60350.010.8649652
CGCGTAT5151.0189979E-810.7216561
CGATAAG40500.010.30500517
CTGGAAT223850.010.159621
GATCGGA39800.010.1450642
CGTTCGC23400.010.02338916
Produced by FastQC (version 0.11.5)