FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24851259
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG66600.033.0467943
GATCGGA67100.032.8079341
ACACGTC74450.032.43419313
TATGCCG72250.030.420746
CGTATGC73150.030.13881544
ACGTCTG82850.029.18686115
CTCGTAT76950.027.65962442
ATCGGAA79650.027.5044042
CACGTCT92050.026.3826614
TCTCGTA81750.025.27602641
ATCTCGT90850.022.97207340
ATGCCGT99350.022.29649247
TCGGAAG99350.022.2935313
CACACGT108700.022.27805712
GCACACG117100.021.56373411
AATCTCG48250.021.30509439
TATCGCG5850.021.22946722
CGTCTGA115450.021.21409616
TTACACG37600.019.90984532
CCGTCTT110550.019.78766850
Produced by FastQC (version 0.11.5)