FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19552416
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40450.020.10834146
TATGCCG43750.018.67382849
CGTATGC46050.017.66248347
CTCGTAT45350.017.41981345
TATCTCG46300.016.98244742
GTCACGA49150.016.20637930
CACGAGT51500.015.26541232
TCTCGTA51700.015.07528244
ACACGTC55600.014.75883114
GATCGGA55450.014.6996222
ACGAGTG53200.014.64797933
TCGCTCG26850.014.51055114
AGTCACG54500.014.42566729
ACGTCTG61100.013.71244316
ATCGGAA59950.013.5961623
AGATCGG59450.013.5955051
TCACGAG62600.012.83450531
CACGTCT65750.012.48047215
CGAAGAT65350.012.4737721
ATCTCGT64700.012.25945443
Produced by FastQC (version 0.11.5)