FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24357956
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA359900.14775459812802028TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG151600.036.34097745
TATGCCG156900.035.61800448
ACGTCCG162500.034.77345732
CGTATGC159500.034.670546
CGTCCGC165800.033.83089433
CTCGTAT165850.033.30172344
ACACGTC169700.033.18157213
AGTCACG168950.032.9762828
GATCGGA174350.032.936451
TCTCGTA166150.032.1830143
GTCCGCA174200.032.00185434
ACGTCTG176500.031.88259115
CGCACAT168050.031.77776337
GTCACGT174450.031.7586429
ATCGGAA181650.031.684052
TCACGTC177750.031.28547530
CACGTCT181600.031.00722914
ATGCCGT183600.030.21279349
ATCTCGT176600.030.08329442
CACGTCC192150.029.2281531
Produced by FastQC (version 0.11.5)