FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24357956
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA86000.032.05702650
GCCGTAT89300.031.3352848
GTCGCCG93450.029.94685445
CGCCGTA93850.029.74534247
CATACGA19650.029.6522315
GCGTCGT99850.028.6977412
TGGTCGC97700.027.65744243
AGATCGG107300.027.3377191
TCGCCGT102300.026.85079646
CCGTATC103750.026.80508249
GGTCGCC115250.024.49252144
GCATACG26950.024.43474414
CGGCATA27050.024.09291312
AGCGTCG121450.023.7047111
GTATCAT118150.023.62560851
GTAGATC124150.023.4990332
GATCGGA127000.023.282212
AGAGCGT125550.023.259769
CGGTGGT119750.022.93118540
CGTCGTG127100.022.62964213
Produced by FastQC (version 0.11.5)